„Alpha“ organizacijos konferencija Šveicarijoje

Rugsėjo 15-17 dienomis, Šveicarijos mieste Luzern`e vyko „Alpha“ organizacijos, vienijančios reprodukcinės medicinos mokslininkus, konferencija, vykstanti kas du metus. Konferencijoje dalyvavo „Vaisingumo klinikos“ embriologė Živilė Čerkienė – Europos žmogaus vaisingumo ir embriologijos draugijos „ESHRE“ narė. Ji pristatė pranešimą „Tirpus žmogaus leukocitų antigenas G (S HLA-G) ankstyvų embrionų kultūrose po dirbtinio apvaisinimo procedūrų“, kurį parengė kartu su mūsų klinikos gydytoja akušere – ginekologe, taip pat ESHRE organizacijos nare – Audrone Usoniene ir VU imunologijos instituto daktare  Audrone Eidukaite.

Ž.Čerkienės skaitytas pranešimas anglų k.:
 
S HLA-G in early human embryo culture after ART
Cerkiene, Z¹ ², Eidukaite, A¹, Usoniene, A.²
 
¹ VU Institute of Immunology, Vilnius, Lithuania
 
² Vaisingumo klinika, Vilnius, Lithuania
 
Introduction: HLA-G is a non-classical HLA class I molecule, that can be expressed in membrane bound or soluble form and is well known for it tolerogenic properties. Increasing interest is now being addressed to the soluble forms, because they might have prognostic properties in implantation and pregnancy process. The aim of our study is to evaluate correlation between embryo cleavage, morphology and  s-HLA-G levels, to estimate the impact of s-HLA-G concentration to pregnancy outcome.

Materials and methods: The study was performed on a group of infertile patients with various infertility indications. IVF or ICSI procedure was used for insemination. After 72 h, on the day of transfer, embryo morphology was evaluated, ET according to embryo morphology was performed ,supernatants of transferred embryos were collected and stored at –90 ºC . For the quantitative measurement of soluble forms of Human Leukocyte Antigen –G in cell culture supernatant we used ELISA method. The ELISA kit from EHBIO Praha measures total sHLA-G and components are ready –to-use. The analysis of results was performed using the Statistics Package for Social Sciences SPSS 12.0.
 
Results: A total of 16 couples participated in this study. In all, 133 embryo culture supernatants were tested for s HLA-G. Soluble form of human leucocyte antigen G was detected in 12 (9.0 %) embryo culture samples (range 0.4-20.5 IU/ml). The production of human leukocyte antigen did not depend on morphological criteria of embryo. No significant differences were found between s HLA-G concentration in patients from IVF and ICSI groups. s HLA-G levels did not differ significantly due to the embryo cleavage rate and pregnancy outcome.
 
Conclusions: day 3 embryos produce s HLA-G to surrounding medium; the level of those soluble molecules can be detected using ELISA. Concentration of human leukocyte antigen did not correlate with number of blastomere and with morphological criteria of embryo. S HLA-G might be one of markers of embryo implantation potential after IVF/ICSI procedures as a factor, which helps to achieve and maintain pregnancy, but further investigations are required.